DETAILS品牌介紹

Bioss lnc.

Bioss lnc.為世界領先的抗體製造商,擁有自主研發抗體技術,致力於提供各類高品質單/多株抗體。目前已發展出超過數萬種以上抗體與十萬種以上相關的螢光標定抗體,其中包含磷酸化、乙醯化、甲基化等修飾抗體。Bioss抗體產品除了已出現在數千篇高品質SCI文章中,實驗應用眾多,如:WB、IHC、IF、ICC、FCM等,滿足各類實驗需求。Bioss產品已遍佈全球多個國家和地區,並已成為數家國際知名抗體公司的OEM合作夥伴,獲得了全世界眾多生命科學領域科研人員的認可。

產品詳細介紹Product Introduction


一級抗體


Bioss Inc生產萬種以上抗體,以及數十萬種以上螢光標定抗體。其中標定抗體多達15種以上不同酵素與螢光選擇,領域包含:Cancer、Stem cell、Virus & Bacteria、Autophagy、Neuroscience、Immunology、Cardiac markers等。此外,抗體適用於多種實驗應用,例如: WB、IHC、IF、ICC、FCM、ELISA等。除了兔子多株抗體,Bioss也致力生產ABBY系列兔子單株抗體,提供客戶更優質的抗體選擇。

 
 

NIH/3T3 cells were stained with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

 


Paraformaldehyde-fixed, paraffin embedded human kidney section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

 


MCF-7 cells were stained with NFKB Polyclonal Antibody, Unconjugated(bs-10037R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


【抗體螢光種類】 
 
FITC 495 / 519 Green
Alexa Fluor® 488 499 / 519 Green
lexa Fluor® 555 553 / 568 Orange
PE 480,565 / 578 Orange
Cy3 512,559 / 570,615 Orange
Alexa Fluor® 594 590 / 617 Orange
Alexa Fluor® 647 650 / 665 Red
Cy5.5 675 / 694 Red
Cy5 625,650 / 670 Red
Cy7 743 / 767 Far Red
Alexa Fluor® 680 679 / 702 Near Red
Alexa Fluor® 750 749 / 775 Near IR
HRP    
Biotin    

 二級抗體


高專一性的二級抗體能辨認多種物種,
包含小鼠、大鼠、兔子、人類、豬、禽、犬等,同時多達12種以上螢光標定組合。


Isotype Control


Bioss擁有多種未標定與標定螢光物質的isotype control antibody,為實驗排除非特異性結合(non-specific binding)所造成的干擾,提供良好的陰性對照組,作為分析時陽性、陰性的最佳依據標準。