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【熱門】Santa Cruz-BCA Protein Assay Kit

【Santa Cruz-BCA Protein Assay Kit】
CAT.# sc-202389
Datasheet/SDS
 
  • Bicinchoninic Acid (BCA) Protein Assay kit is used to determine protein concentration in an unknown sample
     
  • At room temperature, Cysteine, Cystine, Tryptophan, and Tyrosine residues reduce Cu2+ to Cu1+. At higher temperatures (37ºC to 60ºC), peptide bonds in protein also perform this reduction, which produce results that correlate more strongly to protein quantity than to the presence of specific residues
     
  • Each Cu1+ ion forms a complex with two BCA molecules, creating a purple-colored product that absorbs light at 562 nm
     
  • Absorbance data from unknown samples can be plotted against a standard curve and concentration of unknown samples can thus be calculatedEach kit contains: Reagent A (1 L), Reagent B (25 ml), 2mg/ml BSA Standard (25 ml)Our datasheet recommends storage at room temp. However, it may be a good idea to refrigerate the kit to extend the shelf life in order to guarantee it will be stable for 1 year following shipment
     
  • References
    1. LOWRY, O.H., ROSEBROUGH, N.J., FARR, A.L. and RANDALL, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193(1): 265-275. PMID: 14907713
    2. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J. and Klenk, D.C. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150(1): 76-85. PMID: 3843705
    3. Madej, J. and Klimek, R. 1988. Colposcopic observations of the results of immuno-potentialization within the uterine cervix. Ginekol. Pol. 59(3): 147-150. PMID: 3215570
    4. Brown, R.E., Jarvis, K.L. and Hyland, K.J. 1989. Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal. Biochem. 180(1): 136-139. PMID: 2817336